Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis

Figure 4 Sirtuin 1 negatively regulates endoplasmic reticulum stress-mediated apoptotic pathways in Caco-2 monolayers. A: The protein levels of SIRT1 in Caco-2 cells were detected by Western blot; B: The mRNA levels of GRP78 in Caco-2 cells were tested by quantitative real-time PCR; C: The mRNA levels of CHOP in Caco-2 cells were tested by quantitative real-time PCR; D: The protein levels of GRP78, CHOP, caspase 12, caspase 9, and caspase 3 were detected by Western blot in Caco-2 cells. Data are presented as the mean ± SD. ^bP < 0.01 vs UC; ^cP < 0.001 vs UC. UC: Ulcerative colitis; NAM: Nicotinamide; PCR: Polymerase chain reaction; GRP78: Glucose-regulated protein 78; CHOP: CCAAT/enhancer-binding protein homologous protein; SIRT1: Sirtuin 1.