Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis

Figure 1 Establishment of a coculture model in vitro. A: The levels of secreted IL-1β in the upper chamber supernatants were detected by ELISA; B: The levels of secreted TNF-α in the upper chamber supernatants were detected by ELISA; C: The mRNA expression levels of IL-1β and TNF-α in macrophage-like THP-1 cells and Caco-2 cells were tested by quantitative real-time PCR. Data are presented as the mean ± SD. ^bP < 0.01 vs coculture; ^cP < 0.001 vs coculture. LPS: Lipopolysaccharide; IL-1β: Interleukin-1β; TNF-α: Tumor necrosis factor-α; ELISA: Enzyme-linked immunosorbent assay; PCR: Polymerase chain reaction.