Zinc-α2-glycoprotein 1 attenuates non-alcoholic fatty liver disease by negatively regulating tumour necrosis factor-α

Figure 6 Expression of AZGP1/TNF-α, inflammation, and lipid metabolism regulation by AZGP1 in the non-alcoholic fatty liver disease mouse model. Liver tissue images after HE (A) and Sirius red (B) staining are shown in four mouse groups [control (con), HFD, HFD + CCl₄, and HFD + CCl₄ + AZGP1]. Levels of AZGP1 and TNF-α mRNA (C) and protein (D) were detected in the four mouse groups. IF staining (E) with DAPI (blue) and for AZGP1 (green) and TNF-α (red) was also examined in mice. Serum ALT levels (F) were examined using an ELISA kit in the four mouse groups. IL-1β, IL-6, NF-κB, and MCP-1 mRNA (G) and protein (H) expression levels were detected in the four groups. Representative images of ORO staining (I) are shown for the four groups. SREBP-1c, LXR, FAS, ACC, SCD-1, FXR, PPARα, FATP, CPT-1A, and adiponectin mRNA (J) and protein (K) expression was detected in the four groups. ^aP < 0.05 vs control mice; ^cP < 0.05 vs HFD + CCl₄ mice.