Zinc-α2-glycoprotein 1 attenuates non-alcoholic fatty liver disease by negatively regulating tumour necrosis factor-α

Figure 5 AZGP1 exerts multiple functions by blocking TNF-α in LO2 cells. All experiments were performed in LO2 cells, which were divided into six groups (pEX-3, OV-AZGP1, OV-AZGP1 + OV-TNF-α, sh-CTRL, sh-AZGP1, and sh-AZGP1 + sh-TNF-α). AZGP1, TNF-α, NF-κB, IL-6, PCNA, cyclin D1, Bcl2, and caspase 3 mRNA (A, F) and protein (B, G) expression was detected. ORO staining (C, H) of LO2 cells is shown. Cell viability (D, I) was determined using CCK-8 assays. The rate of apoptotic LO2 cells was tested using flow cytometry (E, J). ^aP < 0.05 vs pEX-3 or sh-CTRL cells; ^cP < 0.05 vs OV-AZGP1 or sh-AZGP1 cells.