Zinc-α2-glycoprotein 1 attenuates non-alcoholic fatty liver disease by negatively regulating tumour necrosis factor-α

Figure 4 Proliferation and apoptosis regulation by AZGP1 in LO2 cells. PCNA and cyclin D1 mRNA (A/E) and protein (B/F) expression was detected in LO2 cells. Cell viability was determined using CCK-8 assays (C/G). Cell cycle and proliferation analyses were conducted by flow cytometry (D/H). Bcl2 and caspase 3 mRNA (I/L) and protein (J/M) expression was detected in LO2 cells. The rate of apoptosis was tested using flow cytometry (K/N). ^aP < 0.05 vs pEX-3 or sh-CTRL cells; ^cP < 0.05 vs pEX-3 + PA or sh-CTRL + PA cells.