High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

Figure 7 Sirt1 inhibits Parp1 expression and acetylation in BNL CL₂ cells. A-D: Parp1 expression and acetylation in cells treated with H₂O₂ for different durarions or at different concentrations; E: The mRNA levels of Parp1 in Sirt1 knockdown cells treated with H₂O₂; F and G: Parp1 expression and acetylation in Sirt1 knockdown cells treated with H₂O₂; H and I: Parp1 expression and acetylation in cells treated with SRT1720, a Sirt1 activator. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t-test. ^aP < 0.05 vs 0 group or negative control, ^bP < 0.01 or negative control, ^dP < 0.01 vs H₂O₂ group, ^fP < 0.01 vs CLv group, ^jP < 0.05 vs shLv group, ^kP < 0.01 vs shLv group.