High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

Figure 6 Sirt1 activity is inhibited by H₂O₂ through Parp1-NAD⁺. A and B: NAD⁺ contents in cells treated with H₂O₂ for different durations or at different concentrations; C: NAD⁺ contents in cells treated with DIQ, a Parp1 inhibitor; D: Sirt1 activity in cells treated with DIQ and NAM, an NAD⁺ inhibitor; E and F: High mobility group box-1 (HMGB1) acetylation in cells treated with DIQ; G: BNL CL2 cells were treated with DIQ or H₂O₂. And then HMGB1 contents in medium determined by ELISA; H: HMGB1 contents in medium determined by ELISA in cells treated with CP or Parp1⁺ transfection; I: Sirt1 activity of cells with Parp1⁺ transfection. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t-test. ^bP < 0.01 vs 0 group or negative control, ^dP < 0.01 vs H₂O₂ group, ^hP < 0.01 vs H₂O₂ + DIQ group, ⁱP < 0.01 vs CP group. CP: Control Plasmid.