High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

Figure 3 H₂O₂ induced high mobility group box-1 translocation and acetylation. A: Confocal microscopy analysis of cells treated with H₂O₂ either for different durations or at different concentrations. (scale bar = 40 μm). The white arrow shows the translocation of high mobility group box-1 (HMGB1) to the cytoplasm; B and C: HMGB1 expression determined by Western blot; D and E: Acetylated HMGB1 determined by immunoprecipitation. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t-test. ^aP < 0.05 vs 0 group, ^bP < 0.01 vs 0 group. HMGB1: High mobility group box-1.