High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

Figure 1 Hepatocellular injury in mice treated with a high-fat diet plus single binge alcohol. A: Serum levels of alanine aminotransferase and the levels of H₂O₂ and catalase in C57BL/6 mice fed a control diet for 12 wk or a high-fat diet plus single binge alcohol (n = 12); B: Hematoxylin-eosin stained images (scale bar = 10 μm); C: High mobility group box-1 (HMGB1) staining (scale bar = 20 μm). The black arrow shows HMGB1 translocation; D and E: Western blot analysis for Parp1, Sirt1, and HMGB1 in whole tissue and HMGB1 in the cytoplasm or in nucleus. HMGB1 acetylation was detected by immunoprecipitation; F: The mRNA expression of Parp1 and Sirt1 by real-time PCR and liver NAD⁺ content. All data were presented as mean ± SD. Statistical analysis was done by the Student’s t-test. ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.01 vs control. HFD/etOH: High-fat diet plus single binge alcohol; ALT: Alanine aminotransferase; HMGB1: High mobility group box-1; IP: Immunoprecipitation.