LncRNA-ATB promotes autophagy by activating Yes-associated protein and inducing autophagy-related protein 5 expression in hepatocellular carcinoma

Figure 5 LncRNA-ATB induces autophagy by regulating autophagy-related protein 5 expression. A and B: The mRNA expression of autophagy-related genes involved in autophagy was examined by real-time PCR in hepatocellular carcinoma (HCC) cells transfected with lncRNA-ATB; C and D: The protein levels of ATG5 were analyzed in HCC cells transfected with lncRNA-ATB by Western blot; E and F: RIP-derived RNA was evaluated by real-time PCR. Data are expressed as the mean ± SD of three independent experiments. Statistical significance is indicated at ^aP < 0.05 or ^bP < 0.01. ATG3: Autophagy-related protein 3; ATG5: Autophagy-related protein 5; ATG7: Autophagy-related protein 7; ATG10: Autophagy-related protein 10; ATG12: Autophagy-related protein 12; ATG16L: Autophagy-related protein 16L; HCC: Hepatocellular carcinoma; RIP: RNA immunoprecipitation; LncRNA-ATB: LncRNA-activated by transforming growth factor beta.