LncRNA-ATB promotes autophagy by activating Yes-associated protein and inducing autophagy-related protein 5 expression in hepatocellular carcinoma

Figure 4 LncRNA-ATB promotes autophagy through modulation of Yes-associated protein activation. A and B: The protein levels of total Yes-associated protein (YAP) and p-YAP in hepatocellular carcinoma (HCC) cells transfected with pcDNA3.1-lncRNA-ATB were measured by Western blot; C and D: The nuclear localization of YAP was detected in transfected HCC cells by immunofluorescence; E and F: Knockdown of YAP in HCC cells transfected with si-YAP (si-YAP 1# and si-YAP 2#) was measured using Western blot; G and H: HCC cells were co-transfected with pcDNA3.1-lncRNA-ATB and si-YAP, and the protein levels of LC3 were determined by Western blot. Yes-associated protein: YAP; HCC: Hepatocellular carcinoma; LncRNA-ATB: LncRNA-activated by transforming growth factor beta.