LncRNA-ATB promotes autophagy by activating Yes-associated protein and inducing autophagy-related protein 5 expression in hepatocellular carcinoma

Figure 3 LncRNA-ATB promotes autophagy in hepatocellular carcinoma cells. A and B: Hepatocellular carcinoma (HCC) cells transfected with pcDNA3.1-lncRNA-ATB were subjected to Western blot analysis to detect LC3-II/LC3-I expression, with and without bafilomycin A1 treatment (20 μmol/L for 6 h); C and D: Immunofluorescence of LC3 puncta (green) was measured in HCC cells transfected with pcDNA3.1-lncRNA-ATB or treated with rapamycin; E and F: Electron microscopy identified highly visible autophagosomes and autolysosomes in HCC cells overexpressing lncRNA-ATB compared to negative control cells. HCC: Hepatocellular carcinoma; LncRNA-ATB: LncRNA-activated by transforming growth factor beta.