Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR-1273a

Figure 2 Silencing of circPIP5K1A promotes cell death. A: RT-qPCR detection of circular RNA PIP5K1A expression in COLO320DM cells transfected with siRNA1, siRNA2, or siRNA3 in the absence or presence of RNase R; B: RT-qPCR detection of PIP5K1A mRNA level in COLO320DM cells transfected with siRNA1, 2, and 3 in presence or absence of RNase R; C: Cell death rate in COLO320DM cells transfected with siRNA1, 2, or 3 by Hoechst staining. ^aP < 0.05, ^bP < 0.01 vs oligo or control group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A; siRNA: Small interfering RNAs.