Long non-coding RNA highly up-regulated in liver cancer promotes exosome secretion

Figure 5 Long non-coding RNA highly up-regulated in liver cancer mediates secretion of exosomes from hepatocellular carcinoma cells via miR-372-3p /Rab11a. A: Sequence of binding sites between HULC and miR-372-3p; B: Dual-luciferase reporter assay for miR-372-3p and HULC; C: Relative expression of miR-372-3p after HCC cells were transfected with HULC inhibitors or plasmids causing HULC overexpression; D: Sequence of binding sites with Rab11a and miR-372-3p; E: Dual-luciferase reporter assay for miR-372-3p and Rab11a; F: Relative expression of Rab11a after HCC cells were transfected with miR-372-3p-siRNA or plasmids causing miR-372-3p overexpression. Error bars stand for the mean ± SD of at least triplicate experiments. ^aP < 0.05 vs WT-HULC-NC group; ^bP < 0.05 vs NC group; ^cP < 0.01 vs NC group; ^dP < 0.05 vs WT-Rab11a -NC group; ^eP < 0.05 vs NC group; ^fP < 0.01 vs NC group. HULC: Highly upregulated in liver cancer; NC: Normal control group; HULC: HULC over-expression plasmid group; HULC-siRNA: HULC low-expression plasmid group; miRNA-372-3p: miRNA-372-3p over-expression plasmid group; miR-372-3p-siRNA: miR-372-3p low-expression plasmid group; WT-HULC: HULC wild type luciferase reporter plasmid group; MUT-HULC: HULC mutant luciferase reporter plasmids group; WT-Rab11a: Rab11a wild type luciferase reporter plasmid group; MUT-Rab11a: Rab11a mutant luciferase reporter plasmids group.