Long non-coding RNA highly up-regulated in liver cancer promotes exosome secretion

Figure 4 Long non-coding RNA highly up-regulated in liver cancer induces hepatocellular carcinoma cell proliferation and invasion and inhibits apoptosis in vitro. A: Proliferation of hepatocellular carcinoma (HCC) cells transfected with HULC inhibitors or plasmids causing HULC overexpression; B: Apoptotic pictures of HCC cells (×100). The blue fluorescence is DAPI nuclear staining and green fluorescence is the apoptotic cell. The index of apoptosis is the number of apoptotic cells/total number of visual field × 100%; C: Column chart of index of apoptosis in different groups; D: Effect of HULC on invasion of HCC cells evaluated bTranswell assay (×100); E: Column chart of number of invasive HCC cells in different groups. Error bars stand for the mean ± SD of at least triplicate experiments. ^aP < 0.05 vs HULC-NC 24 h group; ^bP < 0.05 vs HULC-NC 36 h group; ^cP < 0.05 vs HULC-NC 48 h group; ^dP < 0.05 vs HULC-NC 72 h group; ^eP < 0.05 vs NC group; ^fP < 0.05 vs HULC-NC group; ^gP < 0.01 vs NC group; ^hP < 0.01 vs HULC-siRNA-NC group; ⁱP < 0.05 vs NC group; ^jP < 0.05 vs HULC-NC group; ^kP < 0.05 vs NC group; ^lP < 0.05 vs HULC-siRNA-NC group. HULC: Highly upregulated in liver cancer; NC: Normal control group; HULC: HULC over-expression plasmid group; HULC-NC: HULC over-expression normal control plasmid group; HULC-siRNA: HULC low-expression plasmid group; HULC-siRNA-NC: HULC low-expression control plasmid group.