Copyright
©The Author(s) 2019.
World J Gastroenterol. Sep 21, 2019; 25(35): 5300-5309
Published online Sep 21, 2019. doi: 10.3748/wjg.v25.i35.5300
Published online Sep 21, 2019. doi: 10.3748/wjg.v25.i35.5300
Table 1 Primer sequences used for RT-qPCR and siRNA
| RNA | Primers sequences |
| circPIP5K1A | F: 5‘-AGATTCCCTAACCTCAACCAGA-3' |
| R: 5‘-CGAATGTTCTTGCCACCTGC-3' | |
| PIP5K1A | F: 5‘-CCTCATGCAAGATTTCTACGTGG-3' |
| R: 5‘-GGCCGGATACCAAATAGCTCC-3' | |
| GAPDH | F: 5‘-AGAAGGCTGGGGCTCATTTG-3' |
| R: 5‘-AGGGGCCATCCACAGTCTTC-3' | |
| U6 | F: 5‘-AGGGGCCATCCACAGTCTTC-3' |
| R: 5‘-AACGCTTCACGAATTTGCGT-3' | |
| circ-PIP5K1A siRNA-1 | 5'-UUCUUCUAAGGGAUUGGAGUU-3' |
| circ-PIP5K1A siRNA-2 | 5'-UUCUAAGGGAUUGGAGUUGGU-3' |
| circ-PIP5K1A siRNA-3 | 5'-UAAGGGAUUGGAGUUGGUCUU-3' |
| Relative si-NC | 5'-AAUUCUCCGAACGUGUCACGU-3' |
- Citation: Zhang Q, Zhang C, Ma JX, Ren H, Sun Y, Xu JZ. Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR-1273a. World J Gastroenterol 2019; 25(35): 5300-5309
- URL: https://www.wjgnet.com/1007-9327/full/v25/i35/5300.htm
- DOI: https://dx.doi.org/10.3748/wjg.v25.i35.5300