Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations

Figure 2 LNA real-time PCR for identification of hepatitis B virus rtM204I (YIDD) variant and rtM204 (YMDD) wild type. Amplification curves were shown on the left, melting peaks on the right. With YIDD variant DNA templates (A), YIDD specific signals at FAM channel (solid) were detected showing their dominant amplifications with minimal cross signals of amplification generated by a weak YMDD probe cross hybridization and distinct melting temperatures different from those of YMDD DNA with no significant cross signals on melting. For WT DNA templates (B), YMDD specific signals at HEX 6 channel (dashed) were detected showing their exclusive amplifications and distinct T_ms different from those of YIDD with no cross signals. WT: Wild type.