Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations

Figure 1 Primer and LNA-probe positions designed for the detection of hepatitis B virus rtM204I (YIDD) variant and rtM204 (YMDD) wild type. Arrows indicate the primer positions. Underlines indicate the probe positions. The numbers designate the nucleotide position on the hepatitis B virus reverse transcriptase gene sequence. Boldface bases denote the different bases. The box represents the codon and amino acid sequences of the rtM204 (YMDD) wild type and rtM204I (YIDD) variant. This single nucleotide difference is the basis of their discriminative identification by LNA probes in this study. The amino acid sequence is shown as the one-letter amino acid symbols. WT: Wild type.