Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia

Figure 2 Effect of increasing concentration of lipopolysaccharide on enteric ganglia’ HUD+ neurons and GFAP+ glial cells. A: In guinea pig-derived enteric ganglia - lipopolysaccharide (LPS) at 10 µg/mL decreased number of HuD-immunoreactive (HuD-IR) neurons (left columns) and increased proliferation of glial fibrillary acidic protein-immunoreactive (GFAP-IR) glial cells (right columns - HuD-IR neurons LPS10 vs CTRL, 22.3%, ^aP < 0.05; GFAP-IR glial cells LPS10 vs CTRL, +22.2%, ^dP < 0.01); B: Conversely, in pig enteric ganglia the number of glial cells at every LPS concentration tested did not change and was significatively lower compared to ganglionic neurons. ^aP < 0.05. LPS: lipopolysaccharide; GFAP-IR: Glial fibrillary acidic protein-immunoreactive; HuD-IR: HuD-immunoreactive.