Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations

Table 1 Primers and LNA probes developed for identification of hepatitis B virus wild type (YMDD) and rtM204I variants (YIDD) by real-time PCR

Primer/probe	Sequence (5'-3') 1	Tm (°C)2	Target	Channel
Primers (product: 127 bp)
Forward	TGGGCCTCAGTCCGTTTCT	65.4	HBV RT gene
Reverse	TGTACAGACTTGGCCCCCAAWAC	65.2-66.1	HBV RT gene
LNA Probes
YMDD	5' HEX-TAT+A+T+G+G+AT+GAT- 3' IB®FQ	58	YMDD (wild type)	HEX
YIDD	5' 6-FAM-TAT+A+T+T+G+AT+GAT- 3' IB®FQ	53	YIDD	FAM

¹LNA nucleotides are written +A, +C, +T or +G;

²Primer melting temperature was calculated by using LC PDS software V 2.0 and probe melting temperature by https://www.exiqon.com/ls/pages /exiqontmpredictiontool.aspx. T_m: Melting temperature; RT: Reverse transcriptase; HBV: Hepatitis B virus.