Growth arrest-specific gene 2 suppresses hepatocarcinogenesis by intervention of cell cycle and p53-dependent apoptosis

Figure 3 Effect of GAS2 on cell cycle progression. SK-hep1 cells were transfected with pDEST40-GAS2 and pDEST40-CTRL plasmids followed by FACS analysis (FITC/PI). A: Cell populations in different fractions of the cell cycle phase were plotted (^aP < 0.05 vs control); B: The cell population in subG1 phase was determined by flow cytometry (^bP < 0.01 vs control). MIHA cells were transfected with siGAS2 and siCTRL followed by FACS analysis (FITC/PI); C: Cell populations in different fractions of the cell cycle phase were plotted (^cP < 0.05 vs control); D: Cell population in the subG1 phase was determined by flow cytometry. FACS: Fluorescence-activated cell sorting.