Exploring the hepatitis C virus genome using single molecule real-time sequencing

Figure 3 Comparison of coverage curves generated by short-read next-generation sequencing and long-read single-molecular real-time sequencing. A: A coverage curve generated by an IonProton sequencer. Approximately 3120 bp from the NS3 to NS5A region of the hepatitis C virus (HCV) genome from an HCV-infected patient was amplified and long-PCR products were subjected to short-read sequencing. The sequencing depth varies according to genomic location; B: When the same template was sequenced using PacBio RSII sequencer, the coverage curve demonstrates uniform coverage through the NS3 to NS5A regions. HCV: Hepatitis C virus.