MicroRNA-194 inactivates hepatic stellate cells and alleviates liver fibrosis by inhibiting AKT2

Figure 5 iR-194 alleviates liver fibrosis in carbon tetrachloride treated mice. A: Liver inflammation evaluated by H&E staining; B/D: Liver fibrosis evaluated by Sirius Red staining; C/E: IHC of α-SMA was further evaluated in the liver of CCl₄ ± OV-miR194 mice; F: The mRNA levels of Col I, α-SMA, cyclin D1, and AKT2 measured by qPCR; G: The protein levels of Col I, α-SMA, cyclin D1, and AKT2/p-AKT2 measured by Western blot. Bar = 100 μm. ^aP < 0.05 vs the CCl₄ + OV-NC group. H&E: Hematoxylin and eosin; α-SMA: α-smooth muscle actin; Col I: Type I collagen; IHC: Immunohistochemistry.