NKX6.3 protects against gastric mucosal atrophy by downregulating β-amyloid production

Figure 5 NKX6. 3 depletion induces inflammatory cytokine expression in gastric epithelial cells. A: NKX6.3 depletion in HFE-145^shNKX6.3 cells dramatically increased expression of inflammatory cytokines, including interleukin (IL)-1β, IL-6, and IL-8, and cyclooxygenase-2. Shown are the means ± SEM from three experiments. Superscript letter represents significant difference (^aP < 0.005 and ^bP < 0.001). B and C: In HFE-145^shNKX6.3 cells, silencing of apolipoprotein E (ApoE) with siApoE markedly inhibited oligomerization and aggregation of amyloid β and expression of nuclear factor kappa B p-p65 and inflammatory cytokines. Shown are the means ± SEM from three experiments. Superscript letter represents significant difference (^cP < 0.05, ^dP < 0.005 and ^eP < 0.0001). IL: Interleukin; COX-2: Cyclooxygenase-2; ApoE: Apolipoprotein E; Aβ: Amyloid β; NF-κB: Nuclear factor kappa B.