Honokiol-enhanced cytotoxic T lymphocyte activity against cholangiocarcinoma cells mediated by dendritic cells pulsed with damage-associated molecular patterns

Figure 2 Morphology and immunophenotype of human monocyte-derived dendritic cells. Human monocytes were cultured in RPMI1640 medium supplemented with granulocyte-macrophage colony-stimulating factor and interleukin-4 for 6 d. Immature dendritic cells were then stimulated to become mature by adding tumor necrosis factor-α and interferon-γ for 1 d. A: Cell morphology of monocytes. Immature and mature dendritic cells were observed under inverted microscopy at 40×; B: Expressions of CD14 and CD11c human monocytes and mature DCs were presented as percentage of gated cells using flow cytometry CXP software. DCs: Dendritic cells.