MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation

Figure 6 MiR-205 promotes proliferation of pancreatic cancer cells by targeting adenomatous polyposis coli. A: Predicted binding sites of miR-205 in adenomatous polyposis coli (APC). The fragment of wild-type or mutant 3’-UTR of APC was cloned into the luciferase reporter vector; B: Luciferase activities measured at 48 h post transfection; C: APC mRNA expression was decreased in miR-205 mimic treated PANC-1 cells but increased in miR-205 inhibitor treated cells; D and E: The promoting effect of miR-205 on proliferation was reduced in pancreatic cancer cells overexpressing APC, as measured by MTT and colony formation assays. APC: Adenomatous polyposis coli; wt: Wild-type; mut: Mutant.