Mucosal healing progression after acute colitis in mice

Table 4 Kinetics of colonic mRNA expression level normalized to day 7 group (2-ΔΔCt)

	d0	d7	d7 vs d0	d10	d10 vs d7	d13	d13 vsd10	d28	d28 vsd0	Statistical effect (time)
Tight-junction protein
Cldn2	2.41 ± 0.29	1.24 ± 0.21a	↓	2.90 ± 0.15b	↑	1.94 ± 0.17b	-	2.26 ± 0.31b	-	< 0.001
Tjp1	1.45 ± 0.09	1.03 ± 0.08a	↓	1.17 ± 0.10b	↑	1.30 ± 0.06b	-	1.29 ± 0.06b	-	< 0.01
Ocln	2.92 ± 0.37	1.18 ± 0.24a	↓	1.36 ± 0.11a	-	1.96 ± 0.18abc	↑	2.21 ± 0.30bc	-	< 0.001
ECM remodeling
Mmp7	0.58 ± 0.16	1.46 ± 0.40a	↑	5.31 ± 1.37ab	↑	5.21 ± 1.22ab	-	3.69 ± 0.85ab	-	< 0.001
Mmp9	0.13 ± 0.01	1.35 ± 0.24a	↑	1.17 ± 0.27a	-	0.89 ± 0.15a	-	0.55 ± 0.20bcd	-	< 0.001
Timp1	0.05 ± 0.00	1.75 ± 0.49a	↑	0.84 ± 0.25ab	↓	0.41 ± 0.05b	-	0.23 ± 0.06bc	-	< 0.001
Col3a1	0.77 ± 0.07	1.60 ± 0.33a	↑	1.15 ± 0.15a	-	1.20 ± 0.12a	-	0.83 ± 0.16bcd	-	< 0.01
Wisp	0.31 ± 0.02	1.40 ± 0.35a	↑	0.78 ± 0.17ab	↓	0.52 ± 0.04b	-	0.43 ± 0.06b	-	< 0.001
Acta2	1.33 ± 0.17	1.25 ± 0.22	-	0.95 ± 0.16	-	0.79 ± 0.05a	-	0.85 ± 0.11	-	< 0.01
Goblet cell markers and mucins
Tff3	1.80 ± 0.24	1.29 ± 0.32	-	1.05 ± 0.12ad	-	1.62 ± 0.19c	↑	1.87 ± 0.13c	-	< 0.05
Muc2	3.77 ± 0.64	1.10 ± 0.16a	↓	1.72 ± 0.22a	-	3.03 ± 0.18bc	↑	3.38 ± 0.21bc	-	< 0.001
Klf4	2.31 ± 0.13	1.12 ± 0.22a	↓	1.52 ± 0.15a	-	1.73 ± 0.10a	-	2.56 ± 0.24bcd	-	< 0.001
Epithelial repair modulating factors
Igf-1	0.27 ± 0.02	1.23 ± 0.19a	↑	1.14 ± 0.22ad	-	0.77 ± 0.06ac	-	0.55 ± 0.09abcd	-	< 0.001
Il-15	4.59 ± 0.89	1.32 ± 0.51a	↓	1.67 ± 0.18a	-	3.61 ± 0.91bc	↑	4.73 ± 0.86bc	-	< 0.01
Il-22	0.33 ± 0.20	1.59 ± 0.44a	↑	0.36 ± 0.08b	↓	0.47 ± 0.14b	-	0.18 ± 0.04b	-	< 0.001
Il-33	0.08 ± 0.02	1.69 ± 0.43a	↑	0.60 ± 0.11ab	↓	0.43 ± 0.05ab	-	0.26 ± 0.06ab	↑	< 0.001
Saa	1.51 ± 0.27	1.21 ± 0.23	-	1.05 ± 0.16	-	3.40 ± 0.40abc	↑	3.18 ± 0.31abc	↑	< 0.001
Tgf-ß1	0.50 ± 0.03	1.29 ± 0.28a	↑	0.72 ± 0.07b	↓	0.51 ± 0.07b	-	0.43 ± 0.04b	-	< 0.001
Tgf-ß3	1.08 ± 0.11	1.47 ± 0.37	-	0.72 ± 0.10b	↓	0.93 ± 0.12	-	1.16 ± 0.16	-	< 0.01
Inflammatory markers
Il-1ß	0.04 ± 0.01	1.71 ± 0.50a	↑	0.39 ± 0.06ab	↓	0.45 ± 0.07ab	-	0.39 ± 0.09ab	↑	< 0.001
Il-6	0.01 ± 0.00	1.73 ± 0.51a	↑	0.84 ± 0.28ab	↓	0.41 ± 0.08b	-	0.15 ± 0.07b	-	< 0.001
Il-10	0.23 ± 0.03	1.37 ± 0.27a	↑	0.37 ± 0.04b	↓	0.31 ± 0.06b	-	0.25 ± 0.03b	-	< 0.001
Il-13	3.81 ± 0.75	1.26 ± 0.23a	↓	0.82 ± 0.08a	-	0.78 ± 0.10a	-	2.53 ± 0.39bcd	-	< 0.001
Tnf-α	0.13 ± 0.01	1.29 ± 0.22a	↑	0.46 ± 0.05ab	↓	0.50 ± 0.06ab	-	0.47 ± 0.07ab	↑	< 0.001
Ptgs	0.14 ± 0.01a	1.69 ± 0.39b	↑	0.84 ± 0.30c	↓	0.60 ± 0.10 c	-	0.29 ± 0.05a	-	< 0.001

Values are means ± SE (n = 8-12). Means with different superscripts within a row are significantly different.

^aP < 0.05 vs day 0;

^bP < 0.05 vs day 7;

^cP < 0.05 vs day 10;

^dP < 0.05 vs day 13. ECM: Extracellular matrix.