Mucosal healing progression after acute colitis in mice

Table 2 Colon morphometric and inflammatory markers

Parameter	d0	d7	d7 vs d0	d10	d10 vs d7	d13	d13 vs d10	d28	d28 vs d0	Statistical effect (time)
Colon length (cm)	6.5 ± 0.2	4.9 ± 0.3a	↓	5.4 ± 0.2a	-	5.5 ± 0.2a	-	5.7 ± 0.2a	↓	< 0.001
Colon weight/length (mg/cm)	16 ± 0.3	24 ± 0.4a	↑	31 ± 3.0ab	↑	34 ± 3.0ab	-	38 ± 8.0ab	↑	< 0.05
Osmolarity of colonic content	180 ± 8	311 ± 15a	↑	278 ± 8.0a	-	288 ± 13a	-	281 ± 15a	↑	< 0.01
Colonic water content (%)	64.6 ± 2.5	87.5 ± 2.0a	↑	75.5 ± 3.0ab	↓	76.3 ± 1.5ab	-	74.0 ± 1.9ab	↑	< 0.001
IL-1β (ng/mg of total colon protein)	9.4 ± 1.8	14.3 ± 2.4a	↑	13.7 ± 1.8a	-	16.2 ± 2.5a	-	15.4 ± 2.2a	↑	< 0.01
IL-6 (ng/mg of total colon protein)	1.5 ± 0.5	3.6 ± 0.6a	↑	4.2 ± 0.8a	-	4.9 ± 1.2a	-	2.3 ± 0.3bcd	-	< 0.001
MPO activity (UA/mg total colon protein)	1.2 ± 0.2	5.8 ± 1.2a	↑	5.3 ± 0.8a	-	3.1 ± 0.8abc	↓	1.9 ± 0.6bcd	-	< 0.001
Plasmatic LBP (µg/mL)	2.0 ± 0.2	5.8 ± 0.4a	↑	4.5 ± 0.5ab	↓	4.9 ± 0.6ab	-	4.6 ± 0.3c	↑	< 0.001

C57BL/6 male mice were given 3.5% dextran sodium sulfate (DSS) for 5 d in their drinking water in order to induce an acute inflammatory episode. Colon morphometric and inflammatory markers were analyzed at peak colitis (day 7) and during colitis resolution (day 10, day 13 and day 28). Day 0 values correspond to non-DSS treated mice. Values are means ± SE (n = 8-12).

^aP < 0.05 vs day 0;

^bP < 0.05 vs day 7;

^cP < 0.05 vs day 10;

^dP < 0.05 vs day 13.

DSS: Dextran sodium sulfate; LBP: Lipopolysaccharide-binding protein; MPO: Myeloperoxidase activity.