Genetic testing vs microforceps biopsy in pancreatic cysts: Systematic review and meta-analysis

Table 1 Characteristics of studies of molecular analysis included in the analysis

Ref.	Type of study	Number of genes/DNA quantity, ng/microL	Diagnosis	Sample size	Technical success	Adverse events	Diagnostic yield	Surgical cohort	Mean cyst size and range (mm)	Cysts < 3 cm	Cyst location	Specific Cyst Diagnosis	Specific Cyst Diagnosis	High risk cysts	Mucinous low-risk cysts	Benign cysts	Specific cyst type (MA/surgical pathology)
	(study period)	(mean and interval)		(all patients in the study)	(successful molecular analysis)	(major/minor)	(patients with DNA mutations detected)				(H/B/T)Cysts with mutations	(MA/all patients)	(MA/surgical pathology)	(MA/surgical pathology)	(MA/surgical pathology)	(MA/surgical pathology)	IPMN-ADC	IPMN-HG	IPMN-LG	MCN-HG	MCN-LG	SCA	Pseudocyst	NET	Other4
Jones et al[45], 2015	Prospective (Mar 2013-Feb 2014)	39/12.3 (0-1283)	Pathology or cytology	86	79 (only 3 due to NGS failure)	NA	46	10	NA	NA	NA	72/92	5/10	1/6	4/4	0/0	1/4	0/2	4/4	0/0	0/0	0/0	0/0	0/0	0/0
Singhi et al[46], 2016	Retrospective (Jan 2014-May 2015)	6/NA	Pathology or clinicopathologic	225	225	NA	118	41	2.8 (8-115)	152	95/81/49	130/225	32/41	10/14	12/13	10/14	9/92	1/22	12/12	0/0	0/1	2/2	8/8	0/2	0/53
Rosenbaum et al[32], 2016	Retrospective (Mar 2013-Jun 2015)	9/NA	Pathology or clinicopathologic	105	105	NA	67	25	NA	NA	NA	67/1131	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Singhi et al[47], 2017	Prospective (Jan 2014-Ju 2017)	11/6.93 (0.01-248)	Pathology or cytology	642	595	NA	335	102	2.7 (8-210)	NA	320/B+T=306	357/626	77/102	17/29	41/47	19/26	13/13	4/43	39/39	0/2	2/8	2/3	17/17	0/9	0/73
				1058	1004	NA	566 (54%)	178	2,7(8-210)	152		828/965 (86%)	114/153 (74%)	28/49 (62%)	57/64 (89%)	29/40 (73%)	23/26	5/8	55/55	0/2	2/9	4/5	25/25	0/11	0/12

¹113 samples of 105 patients;

²TP53, PIK3CA, PTEN;

³1 ACC;

⁴includes: ACC, retention cyst, lymphoepithelial cyst, epidermoid cyst, and squamoid cyst. MA: Molecular analysis; NA: Non-available data; ACC: Acinar cell cyst; H/B/T: Head body and tail of the pancreas; ADC: Adenocarcinoma; HG: High-grade; LG: Low-grade; NET: Neuroendocrine tumor.