Development and in vitro study of a bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma

Figure 6 Prussian blue staining of Hepa1-6/GPC3 cells treated with four kinds of ultra-small superparamagnetic iron oxide probes. Prussian blue-staining images of blank Hepa1-6/GPC3 cells (A) and Hepa1-6/GPC3 cells treated with 50 µg Fe/mL of (B) USPIO (U), (C) anti-AFP–USPIO (UA), (D) anti-GPC3–USPIO (UG), or (E) anti-AFP–USPIO–anti-GPC3 (UAG). (F) Quantitation of the percentages of blue stained cells. The total counted cell number for the U, UA, UG, and UAG groups was 84, 133, 199, and 119, respectively. USPIO: Ultra-small superparamagnetic iron oxide; AFP: Alpha-fetoprotein; GPC3: Glypican-3; UAG: Anti-AFP–USPIO–anti-GPC3; UA: Anti-AFP–USPIO; UG: Anti-GPC3–USPIO; U: Unlabeled (non-targeted) USPIO.