NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling

Figure 3 NIMA related kinase 2 mediates the activation of ERK/MAPK signaling. A and B: Relative mRNA expression of ERK/MAPK signaling key factors analyzed by quantitative real-time PCR in NIMA related kinase 2 (NEK2) silenced BGC823 and SGC7901 cell lines and negative controls. C: Western blot analysis of ERK/MAPK signaling key factors (ERK, c-JUN, and cyclin D1) and their phosphorylated forms (p-ERK and p-c-JUN) in NEK2 silenced BGC823 and SGC7901 cell lines and negative controls. D: Relative phosphorylation levels of ERK and c-JUN in C were calculated. β-actin was used as the internal control. Values are presented as the mean ± SEM. NEK2: NIMA related kinase 2.