NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling

Figure 1 Expression of NIMA related kinase 2 in tissues and cells of gastric cancer. A: Relative mRNA expression level of NIMA related kinase 2 (NEK2) in gastric cancer and normal tissues (datasets from Oncomine). B: Relative mRNA expression level of NEK2 analyzed by quantitative real-time PCR in 30 pairs of human gastric cancer tissues (Cancer) and adjacent tissues (Normal). (Paired t-test, P < 0.001). C: The mRNA expression level of NEK2 in different grades of cancer samples. Data represents the mean ± SD of three replicates. D: Relative NEK2 mRNA expression level in four gastric cancer cell lines compared to gastric mucosal epithelial cell line (GES1). E: Relative NEK2 protein expression level in different gastric cancer cell lines. β-actin was used as the loading control. NEK2: NIMA related kinase 2.