Sp1 contributes to overexpression of stanniocalcin 2 through regulation of promoter activity in colon adenocarcinoma

Figure 5 The transcription factor Sp1 was involved in the promoter activity of STC2. A: Overexpression of Sp1 and control vector in HCT116 and NCM460 cells and the promoter activity were tested by luciferase reporter assay, ^cP < 0.001 vs Myc-Vector of individual cell group; B: Different Sp1 overexpression plasmids were transfected in SW480 cells, the promoter activity was examined 24 h after transfection, ^cP < 0.001 vs Myc-Vector group; C: Knockdown of expression of Sp1 in HT29 and D: SW480 cell lines with different dose, and reporter activity was conducted after 48 h transfection, ^cP < 0.001 vs sh-Vector. STC2: Stanniocalcin 2.