Sp1 contributes to overexpression of stanniocalcin 2 through regulation of promoter activity in colon adenocarcinoma

Figure 3 The core region of the STC2 promoter was -358/+286 nt and correlated with the protein levels. A: The relative promoter activity of different regions of the STC2 promoter was evaluated by luciferase reporter assay in HT29 cells, ^cP < 0.001 vs pGL3-Basic; B: The relative promoter activity of core region -358/+286 nt and (C: mRNA expression levels were examined in different cell lines, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs HEK293T; D: Western blotting was performed to examine the protein expression of STC2 in different cell lines; E: The relative protein density of panel D was conducted by Quantity One software. ^bP < 0.01, ^cP < 0.001 vs HEK293T. STC2: Stanniocalcin 2.