Systems pharmacology approach reveals protective mechanisms of Jian-Pi Qing-Chang decoction on ulcerative colitis

Figure 4 Jian-Pi Qing-Chang restrains the HIF-1α signalling pathway in bone marrow-derived macrophages. A: The isolating method and culturing conditions of bone marrow-derived macrophages (BMDMs) from wild-type C57BL/6 mice. Mature BMDMs were treated with TNF-α (20 ng/mL) in the presence or absence of either Jian-Pi Qing-Chang (100 μg/mL) or rapamycin (2 μM) for 24 h, or pre-treated with pyrrolidinedithiocarbamate (50 μM) for 30 min; B: The relative mRNA expression in BMDMs was measured by PCR; C-E: The relative protein expression in BMDMs was measured by Western blot; F: Immunofluorescence staining for HIF-1α in BMDMs. Data are presented as the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. BMDMs: Bone marrow-derived macrophages; JPQC: Jian-Pi Qing-Chang; PDTC: Pyrrolidinedithiocarbamate; RAP: Rapamycin.