Prognostic significance of lymphovascular invasion in colorectal cancer and its association with genomic alterations

Figure 3 DCNA classifier for differentiating between colorectal cancers with and without LVI. LVI were distinguished from those without it at a 95.7% accuracy rate by examining seven special DCNAs. The procedures of this analysis were as follows: chr6: 95148725-95193920 was taken as the first criterion. If a tumor showed gain at chr6: 95148725-95193920, it was categorized as a tumor with LVI. Six of 47 tumors were classified into this group. When the DCNA of the tumor was not this case, the second criterion (Loss at chr17: 30770711-30859316) was examined. Five tumors were separated from the remaining 41 tumors at this point. If the tumors showed no loss at chr17: 30770711-30859316, then the third criterion (Gain at chr14: 106113380-106135655) was checked. In this way, the DCNA of the tumor was in turn examined from the 1st to the 7th one. Each step successively sorted a cluster of either group. Finally, all colorectal cancers were divided into either of two groups, tumors with and without LVI. DCNA: DNA copy number alteration; LVI: Lymphovascular invasion.