Trimethylamine N-oxide attenuates high-fat high-cholesterol diet-induced steatohepatitis by reducing hepatic cholesterol overload in rats

Figure 6 Diversity of gut microbiota is restored by trimethylamine N-oxide treatment in rats fed a high-fat high-cholesterol diet. A: The beta diversity of the gut microbiota (n = 4); B: The rarefaction curve of the Shannon measurement (n = 4); C: Index bar of the Shannon measurement (n = 4); D: The rarefaction curve of the Simpson measurement (n = 4); E: Index bar of the Simpson measurement (n = 4); F: Linear discriminant analysis coupled with effect size measurements analysis (n = 4); G: Prediction of the functional genes in the sampled bacterial community using phylogenetic investigation of communities by reconstruction of unobserved states (n = 4). ^aP < 0.05, vs rats fed a chow diet; ^bP < 0.01, vs rats fed a chow diet; ^cP < 0.05, vs rats fed a high-fat high-cholesterol diet; ^dP < 0.01, vs rats fed a high-fat high-cholesterol diet. TMAO: Trimethylamine N-oxide; HFHC: High-fat high-cholesterol.