Extract of Cycas revoluta Thunb. enhances the inhibitory effect of 5-fluorouracil on gastric cancer cells through the AKT-mTOR pathway

Figure 3 The chemosensitization of Cycas revoluta Thunb. natural extract with 5-fluorouracil. A: Clonogenic assays. The error bars represent ± STDEV from n = 3 independent experiments, ^bP < 0.01; B: Dose-response assays of 5-fluorouracil (5-Fu) with the extract; C: The half-maximal effective concentration and the half-maximal lethal concentration of 5-Fu in combination with the extract. The error bars represent ± STDEV from n = 3 independent experiments; D: Western blot analysis of drug-resistance-related proteins. GAPDH was used as a control. Relative expressions are shown in the left histogram, and the error bars represent ± STDEV from n = 3 independent experiments ^aP < 0.05, ^bP < 0.01. 5-Fu: 5-fluorouracil; EC50: Half-maximal effective concentration; LC50: Half-maximal lethal concentration; mTOR: Mammalian target of rapamycin; P-gp: P-glycoprotein.