Functional role of long non-coding RNA CASC19/miR-140-5p/CEMIP axis in colorectal cancer progression in vitro

Figure 5 MiR-140-5p suppresses cancer susceptibility 19-induced enhancement of colorectal cancer cell proliferation and metastasis. A: Growth curves of SW480 and HCT-116 cell lines after being transfected by either miR-140-5p inhibitor or siCASC19 were determined by the Cell Counting Kit-8 (CCK-8) assay. B: Growth curves of SW480 and HCT-116 cell lines after being transfected by either miR-140-5p mimic or CASC19-p were analyzed by the CCK-8 assay. C: The number of invading HCT-116 and SW480 cells was determined by transwell assay after being transfected by either miR-140-5p inhibitor or siCASC19. Scale bars = 20 μm. D: The number of invading HCT-116 and SW480 cells was determined by transwell assay after being transfected with either miR-140-5p mimic or CASC19-p. Scale bars = 20 μm. E: The expression of E-cadherin and vimentin in CRC cells was determined by Western blot after being transfected by either miR-140-5p inhibitor or siCASC19. F: The expression of E-cadherin and vimentin in CRC cells was determined by Western blot after being transfected with either miR-140-5p mimic or CASC19-p. Data are reported as the mean ± SD. ^aP < 0.05. CASC19: Cancer susceptibility; CRC: Colorectal cancer; CEMIP: Cell migration inducing hyaluronidase 1.