Clinical assessment and identification of immuno-oncology markers concerning the 19-gene based risk classifier in stage IV colorectal cancer

Table 1 Association between differential regulation of 19-gene-based risk classifier genes and clinicopathological factors in 60 patients with stage IV colorectal cancer

Variables (No. of patients)	Percentage of tumors with a log22-ΔΔCT value > 1 compared with matched normal tissue (P value)
	GADD45B1	S1PR3	CDKN2B	EGR2	CTGF	SERPINE1	RGS16	RHOU	TIMP1	PHLDA1	IL36RN	SLAMF7	E2F7	DTL	CFB	CDK1	CXCL1	CXCL3	CKS2	TREM1
Mean ± SEM of 2ΔΔCT	0.78 ± 0.74	1.45 ± 4.23	1.64 ± 6.03	3.24 ± 10.88	1.23 ± 1.66	8.19 ± 22.49	10.39 ± 35.49	1.01 ± 1.59	2.90 ± 2.79	5.49 ± 14.95	4.40 ± 15.22	1.17 ± 5.10	9.84 ± 34.23	2.46 ± 4.68	3.29 ± 5.62	2.94 ± 6.51	22.76 ± 75.79	30.55 ± 76.03	2.65 ± 4.14	14.14 ± 71.65
Sex	25.9/24.2	25.9/27.3	22.2/15.2	33.3/39.4	37.0/36.4	74.1/81.8	81.5/69.7	33.3/24.2	74.1/75.8	63.0/66.7	33.3/42.4	3.7/18.2	85.2/84.8	44.4/60.6	70.4/69.7	51.9/60.6	92.6/78.8	96.3/84.8	55.6/75.8	29.6/42.4
Female/male (27/33)	0.56	0.57	0.35	0.42	0.58	0.34	0.23	0.31	0.56	0.49	0.33	0.89	0.63	0.16	0.59	0.34	0.13	0.15	0.08	0.23
Age	23.2/50.0	26.8/25.0	17.9/25.0	37.5/25.0	35.7/50.0	76.8/100	75.0/75.0	28.6/25.0	73.2/100	64.3/75.0	39.3/25.0	10.7/25.0	83.9/100	55.4/25.0	71.4/50.0	58.9/25.0	83.9/100	89.3/100	66.1/75.0	35.7/50.0
≤ 75 vs > 75 (56/4)	0.26	0.71	0.57	0.53	0.47	0.37	0.69	0.68	0.31	0.56	0.5	0.4	0.51	0.26	0.35	0.21	0.51	0.65	0.59	0.47
Comorbidity	20.0/32.0	31.4/20.0	20.0/16.0	42.9/28.0	37.1/36.0	74.3/84.0	80.0/.68.0	34.3/20.0	68.6/84.0	62.9/68.0	45.7/28.0	8.6/16.0	80.0/92.0	60.0/44.0	71.4/68.0	68.6/40.0	82.9/88.0	88.6/92.0	68.6/64.0	37.1/36.0
No/Yes (35/25)	0.22	0.25	0.48	0.18	0.57	0.28	0.22	0.18	0.14	0.45	0.13	0.31	0.18	0.17	0.5	0.03	0.43	0.51	0.46	0.57
Family history	24.0/30.0	28.0/20.0	18.0/20.0	38.0/30.0	36.0/40.0	78.0/80.0	72.0/90.0	28.0/30.0	74.0/80.0	64.0/70.0	42.0/20.0	12.0/10.0	88.0/70.0	56.0/40.0	70.0/70.0	60.0/40.0	88.0/70.0	94.0/70.0	72.0/40.0	36.0/40.0
No/Yes (50/10)	0.48	0.46	0.59	0.46	0.54	0.63	0.22	0.59	0.52	0.51	0.17	0.67	0.16	0.28	0.63	0.21	0.16	0.05	0.06	0.54
Synchronous Lm	33.3/21.4	33.3/23.8	22.2/16.7	33.3/38.1	33.3/38.1	72.2/81.0	83.3/71.4	44.4/21.4	77.8/73.8	66.7/64.3	44.4/35.7	22.2/7.1	72.2/90.5	55.6/52.4	83.3/64.3	72.2/50.0	100/78.6	100/85.7	83.3/59.5	38.9/35.7
No/Yes (18/42)	0.25	0.32	0.43	0.48	0.48	0.33	0.26	0.07	0.51	0.55	0.36	0.11	0.08	0.52	0.12	0.09	0.03	0.1	0.06	0.52
Synchronous Pm	28.9/13.3	26.7/26.7	17.8/20.0	37.8/33.3	35.6/40.0	77.8/80.0	77.8/66.7	31.1/20.0	77.8/66.7	66.7/60.0	40.0/33.3	13.3/6.7	86.7/80.0	57.8/40.0	71.1/66.7	62.2/40.0	88.9/73.3	91.1/86.7	66.7/66.7	40.0/26.7
No/Yes (45/15)	0.2	0.64	0.56	0.51	0.49	0.58	0.3	0.32	0.3	0.43	0.44	0.43	0.4	0.18	0.49	0.11	0.15	0.47	0.62	0.27
AJCC stage	0/25.9	0/27.6	0/11	0/37.9	50/36.2	50/79.3	100/74.1	50/27.6	0/77.6	50/65.5	50/37.9	0/12.1	100/84.5	100/51.7	100/69.0	100/55.2	100/84.5	100/89.7	100/65.5	0/37.9
III/IV (2/58)	0.56	0.53	0.66	0.4	0.6	0.39	0.56	0.49	0.06	0.58	0.62	0.78	0.72	0.28	0.49	0.32	0.72	0.81	0.44	0.4
LVI	10.0/32.5	20.0/30.0	15.0/20.0	25.0/42.5	25.0/42.5	65.0/85.0	65.0/80.0	30.0/27.5	75.0/75.0	45.0/75.0	25.0/45.0	0/17.5	70.0/92.5	35.0/62.5	60.0/75.0	45.0/62.5	75.0/90.0	85.0/92.5	55.0/72.5	30.0/40.0
No/Yes (20/40)	0.05	0.31	0.47	0.15	0.15	0.08	0.17	0.53	0.63	0.02	0.11	0.05	0.03	0.04	0.18	0.16	0.13	0.31	0.14	0.32
PNI	12.0/34.3	32.0/22.9	16.0/20.0	40.0/34.3	32.0/40.0	76.0/80.0	60.0/85.7	36.0/22.9	72.0/77.1	56.0/71.4	40.0/37.1	8.0/14.3	76.0/91.4	52.0/54.3	68.0/71.4	56.0/57.1	76.0/91.4	84.0/94.3	64.0/68.6	24.0/45.7
No/Yes (25/35)	0.04	0.31	0.48	0.43	0.36	0.47	0.02	0.2	0.44	0.17	0.52	0.37	0.1	0.53	0.5	0.57	0.1	0.19	0.46	0.07
CRM involvement	22.4/36.4	26.5/27.3	18.4/18.2	34.7/45.5	38.8/27.3	79.6/72.7	73.5/81.8	30.6/18.2	73.5/81.8	63.3/72.7	36.7/45.5	6.1/36.4	83.7/90.9	51.0/63.6	69.4/72.7	53.1/72.7	85.7/81.8	89.8/90.9	61.2/90.9	30.6/63.6
No/Yes (49/11)	0.27	0.61	0.68	0.37	0.36	0.44	0.44	0.34	0.44	0.41	0.42	0.02	0.47	0.34	0.57	0.2	0.52	0.7	0.05	0.04

¹GADD45B, control gene. Rt: Right; Lt: Left; Re: Rectum; Lm: Hepatic metastasis; Pm: Pulmonary metastasis; AJCC: American Joint Committee on Cancer; LVI: Lymphovascular invasion; PNI: Perineural invasion; CRM: Circumferential resection margin; IHC: Immunohistochemistry.