MicroRNA-596 acts as a tumor suppressor in gastric cancer and is upregulated by promotor demethylation

doi:10.3748/wjg.v25.i10.1224

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2019; 25(10): 1224-1237
Published online Mar 14, 2019. doi: 10.3748/wjg.v25.i10.1224

Table 1 Sequences of primers for quantitative real-time PCR

Name	Sequence (5'-3')
Primers used for mRNA detection
miR-596 (forward)	AAGCCTGCCCGGCTCCT
miR-596 (reverse)	GCTGTCAACGATACGCTACGT
miR-596 (RT)	(GCTGTCAACGATACGCTACGTAACGGCATGACA; GTGTTTTTTTTTTTTTTTTTTTTTTTTC)
U6 (forward)	CGCTTCGGCAGCACATATAC
U6 (reverse)	TTCACGAATTTGCGTG TCAT
U6 (RT)	(GCTGTCAACGATACGCTACGTAACGGCATGACAG; TGTTTTTTTTTTTTTTTTTTTTTTTTG)
PRDX1 (forward)	AAGAAACTCAACTGCCAAGTG
PRDX1 (reverse)	CAGCCTTTAAGACCCCATAAT
GAPDH (forward)	CGGATTTGGTCGTATTGGG
GAPDH (reverse)	CTGGAAGATGGTGATGGGATT

PRDX1: Peroxiredoxin 1; MiR-596: MicroRNA-596; GAPDH: Glyceraldehyde phosphate dehydrogenase.

Citation: Zhang Z, Dai DQ. MicroRNA-596 acts as a tumor suppressor in gastric cancer and is upregulated by promotor demethylation. World J Gastroenterol 2019; 25(10): 1224-1237
URL: https://www.wjgnet.com/1007-9327/full/v25/i10/1224.htm
DOI: https://dx.doi.org/10.3748/wjg.v25.i10.1224