Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

Figure 3 lncRNA RP4 functions as an miR-7-5p decoy in colorectal cancer cells. A: The predicted miR-7-5p binding sites on the SH3GLB1 and lncRNA RP4 transcript. B: LncRNA RP4 overexpression and knockdown, respectively, decreased and increased the expression of miR-7-5p in SW480 cells. C: Correlation analyses revealed a linear association between the expression of lncRNA RP4 and miR-7-5p, with an r² value of 0.482. D: SW480 cells were transfected with an miR-7-5p mimic and inhibitor, and cell proliferation was evaluated by the CCK-8 assay. miR-7-5p overexpression and knockdown increased and decreased cell proliferation, respectively. E. Real-time quantitative PCR showed that miR-7-5p overexpression and knockdown, respectively, decreased and increased SH3GLB1 expression level in SW480 colorectal cancer cells. ^aP < 0.05, ^bP < 0.01, and ^eP < 0.001 for between-group comparisons.