Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

Figure 1 lncRNA RP4 regulates proliferation, tumor growth, and early apoptosis in colorectal cancer cells. Lentivirus-mediated lncRNA RP4 overexpression and knockdown were performed in the colorectal cancer line SW480, and cell proliferation, tumor growth, and early apoptosis were examined. A: Cell proliferation was examined by the CCK-8 assay. lncRNA RP4 overexpression and knockdown were shown to decrease and increase cell proliferation, respectively. B: Tumor growth was evaluated by tumor volume change. lncRNA RP4 overexpression and knockdown were shown to significantly decrease and increase tumor volume, respectively, at weeks 14, 21, and 28. C: Flow cytometry assessment of early apoptosis. lncRNA RP4 overexpression and knockdown increased and decreased early apoptosis, respectively, in colorectal cancer. D: Early apoptosis quantification. ^eP < 0.001 for between-group comparisons.