Mismatched effects of receptor interacting protein kinase-3 on hepatic steatosis and inflammation in non-alcoholic fatty liver disease

Figure 2 Effect of receptor interacting protein kinase-3 deletion on fat synthesis. A-J: Quantitative real-time PCR analysis showed an increased expression of RIP3 the WT-HF group after HF diet feeding. Interestingly, very-low-density lipoprotein secretion markers including apolipoprotein-B, microsomal triglyceride transfer protein, protein disulfide isomerase, and X-box binding protein-1 were decreased in the RIP3KO-HF group compared to the WT-HF group. The differences in SREBP1c, FAS, CD36, DGAT, and PPAR-α were not definite. ^aP < 0.05 by Mann-Whitney U test, compared to NC diet fed WT group; ^bP < 0.05 by Mann-Whitney U test, compared to NC diet fed RIP3-KO group; ^cP < 0.05 by Mann-Whitney U test, compared to HF diet fed WT group. HF: High fat; KO: Knockout; NC: Normal chow; WT: Wild-type; RIP3: Receptor interacting protein kinase-3; VLDL: Very-low-density lipoproteins; ApoB: Apolipoprotein-B; MTTP: Microsomal triglyceride transfer protein; PDI: Protein disulfide isomerase; XBP1: X-box binding protein-1; SREBP1c: Sterol regulatory element-binding protein-1c; FAS: Fatty acid synthase; CD36: Cluster of differentiation-36; DGAT: Diglyceride acyltransferase; PPAR-α: Peroxisome proliferator-activated receptor alpha.