Novel screening test for celiac disease using peptide functionalised gold nanoparticles

Figure 1 Schematic representation of preparation of peptide coated AuNPs. A: Maleimide groups of the linker reacted specifically with free (reduced) sulfhydryl’s in the peptide sequence to form stable thio-ether bonds; B: NeutrAvidin was coated on the surface of the AuNPs to obtain NeutrAvidin-AuNP particles; C: The biotin end of the linker interacted with the NeutrAvidin-AuNPs resulting in the formation of peptide coated AuNPs.