MicroRNA-15a - cell division cycle 42 signaling pathway in pathogenesis of pediatric inflammatory bowel disease

Figure 2 Tumor necrosis factor-α induced microRNA-15a upregulation and cell division cycle 42 downregulation in Caco-2 cells. Caco-2 cells were stimulated with tumor necrosis factor-α (TNF-α) (35 ng/mL) (TNF-α group) or medium with no TNF-α (control) for 60 h. Quantitative reverse transcription polymerase chain reaction (commonly known as qRT-PCR) was used to evaluate the relative mRNA expression of miR-15a in A: the control and TNF-α groups; B: qRT-PCR was used to evaluate the relative mRNA expression Cdc42 in the control and TNF-α groups (^aP < 0.05 vs control). Cdc42: Cell division cycle 42; miR: MicroRNA; TNF-α: Tumor necrosis factor-α.