Glucocorticoid receptor regulates expression of microRNA-22 and downstream signaling pathway in apoptosis of pancreatic acinar cells

Figure 2 Upregulation of microRNA-22 promotes the apoptosis of AR42J cells by suppressing the PI3k/Akt signaling pathway. A: MicroRNA-22 expression level; B: Erb-b2 receptor tyrosine kinase 3 (ErbB3) mRNA expression level; C: ErbB3 protein expression level; D: The apoptosis rate of AR42J cells induced with caerulein (Cae) after transfection; E: Amylase levels in the medium; F: Western blot analysis of the levels of PI3k, p-PI3k, Akt, p-Akt, and apoptosis-associated proteins in AR42J cells. Data were obtained from three independent experiments performed in triplicate and are shown as the mean ± SD. ^NSP > 0.05, ^cP < 0.001 vs miRNA NC or miRNA NC + Cae groups. ErbB3: Erb-b2 receptor tyrosine kinase 3; Cae: Caerulein; miRNA: MicroRNA; miR-22: MicroRNA-22.