Neonatal rhesus monkeys as an animal model for rotavirus infection

Figure 2 Immunofluorescence of rotavirus antigen in the jejunum of neonatal rhesus monkeys inoculated with SA11 or medium without serum. A-C: The jejunum of neonatal rhesus monkeys inoculated with medium without serum at 3 dpi; D-F: Jejunum of neonatal rhesus monkeys inoculated with 10⁸ PFUs of SA11/monkey at 3 dpi. The glass slides were incubated with goat anti-rotavirus (RV) polyclonal antibody and then incubated with rabbit anti-goat IgG antibody labeled with FITC (green). Cell nuclei are shown with DAPI staining (blue). White arrows indicate representative RV-positive cells. Magnification, × 20. Bar: 50 μm.