Effects of alkaline-electrolyzed and hydrogen-rich water, in a high-fat-diet nonalcoholic fatty liver disease mouse model

Table 4 Expression of genes relevant to nonalcoholic fatty liver disease from hepatocytes isolated from mice drinking regular water or hydrogen-rich water after palmitate overloading in vitro

	RW		HRW		P value
	M	Sd	M	Sd
TNF-α	9.16a	1.90	5.71b	4.59	0.036
IL-6	3.2a	0.87	2.24b	0.64	0.002
CRP	0.96	0.28	1.20	0.34	0.065
SREBP-1c	0.96	0.12	1.05	0.19	0.196
ACC1	0.84	0.18	0.86	0.14	0.902
SOD	1.07	0.34	1.30	0.56	0.184
AdipoR2	0.80	0.08	0.78	0.18	1.000
Adipo	1.00	0.30	0.79	0.30	0.097

Each value is the average of hepatocytes obtained from 4 different mice after normalization with keeping genes (β2m, β-actin). Statistically significant difference marked by different letters (Kruskal-Wallis, Mann Whitney test).