Development of a novel rat model of heterogeneous hepatic injury by injection with colchicine via the splenic vein

Figure 2 Histopathological changes of the liver (× 100). H&E staining of sections of the left lobe (1^st column) and right lobe (2^nd column) of the liver, and Masson’s trichrome staining of sections of the left lobe (3^rd column) and right lobe (4^th column) of the liver were performed in the control group (1^st row) and at each time point after injection of colchicine (2^nd to 7^th rows). No obvious pathological changes were observed in the control group. The hepatic injury was different between the left and right lobes at each time point. At day 3 after colchicine injection, there was massive inflammatory cells infiltration, hepatocellular edema, and mild liver necrosis. At day 14, reduced inflammation and increased necrosis were observed, while fibrosis was not detected. At week 4, cholestasis and early fibrosis were observed. At weeks 8 and 12, there was further fibrosis.